High Stem Cell Recovery

At LifeCell, the processing technology that is used is customized to the volume of cord blood sample obtained. The volume of stem cells separated from the sample is higher at LifeCell when compared to automated techniques. Our technology also ensures lower red blood cell contamination, thereby achieving the best quality harvest of stem cells from the cord blood sample.

The efficiency of cord blood processing is dependent on largely two parameters namely

  1. The recovery of nucleated cells and also
  2. The reduction of the red blood cells.

Below is a comparison of LifeCell’s Proprietary Processing Technique versus other Generally used Processes.

Median Recovery of Total Nucleated Cell Recovery

TNC Recovery
  • 80% LifeCell (n=36489)
  • 76.76% AXP(n=1000)
  • 78.81% Sepax(n=670)

LifeCell’s proprietary process personalized for cord blood of various volumes ensures that we achieve a median recovery of ~80%1, which is higher than other automated techniques such as the AXP or Sepax2.

Our process enables us to have an internal control system (which is not possible in Sepax)3 which ensures that samples having less than 70% TNC recovery can be re-processed to retrieve the cells which were lost in the initial processing due to clots etc, thereby reducing loss of precious stem cells.

Red Blood Cell Reduction (~10% Better)

RBC Reduction
  • 95.40% LifeCell (n=36489)
  • 88.28% AXP(n=1000)
  • 88.32% Sepax(n=670)

LifeCell’s proprietary process for red cell reduction ensures that we obtain>95%1 reduction in red cells from the original levels, about 10% higher than other automated techniques. Importantly only 0.1% of the samples have haematocrit values >40% at LifeCell versus others such as 1.2% reported for AXP and 8.9%reported for Sepax2.

References :
  1. LifeCell’s in-house cord blood processing data as presented at the 22nd International Society for Cellular Therapy (ISCT) meeting held in Singapore in May 2016 (Analysis of 36489 samples).
  2. Solves P, Planelles D, Mirabet V, Blanquer A, Carbonell-Uberos F. Qualitative and quantitative cell recovery in umbilical cord blood processed by two automated devices in routine cord blood banking: a comparative study. Blood Transfus. 2013;11:405–411
  3. Sepax Cell Processing System: Operator's Manual.