The samples are tested to ensure viability, cell count, sterility and to check for presence of transmittable diseases. The test results show whether a sample is suitable for storage and if there are any disease conditions detected, parents will be notified.
Ideally, the umbilical cord samples should be processed within 48 hours of collection to maintain the viability of the stem cells. Thus, transportation time is crucial for cord blood, tissue and maternal blood samples. At LifeCell, the samples are transported through specialized logistics provider to ensure a speedy and x-ray free transportation.
LifeCell offers a personalized technique called âProprietary Personalized Processingâ in which the stem cells are isolated from the cord blood by centrifuge method and preserved in a cryo preservation chamber. This process is customized based on the volume of the sample and differs from others in a way, the samples are centrifuged and stem cells are separated. To know more about the process, read here. After testing for quality, the cord tissue is reduced to smaller portions and subjected to an explant cell culture process in order to isolate the stem cells from the tissue and cryo-preserved.
No. At LifeCell, we give priority to every client, and hence there are 3 shifts to process the incoming samples. Each shift has 4-5 well experienced technicians to process over 60 samples per shift. Thus, your sample will be processed well ahead of time with precision.
After initial processing in the lab, technologist will be able to confirm the status of contamination, if any. However, the chances of samples having contamination are very rare.
Apart from the LifeCell's personalized closed bag processing method, there are other methods such as AXP, Sepax etc., which yields lesser stem cells comparatively.
The efficiency of stem cell processing depends on maximum recovery of nucleated cells and reduction of RBCs from the sample.
LifeCell's proprietary process can recover over 85% of total nucleated cells which is about 10% higher than other automated processes like AXP and Sepax. It also ensures over 95% of reduction in red blood cells which is also about 10% higher than other automated methods. Thus, LifeCell has better processing technology when compared to other cord blood banks.
The stem cells from cord blood and tissue are stored in well protected cryopreservation chambers. The sample bags are kept inside the aluminum canisters which are placed within the cryo-cylinders and preserved for long term storage at -196 degrees. The samples are stored in dual storage sites: Chennai and Gurgaon for additional protection.
No. Upon arrival, each sample is carefully examined in the sample receipt area and bar-coded with accuracy to track them. The bar-coded samples are then sent for testing and processing without delay. The sample data are generated by an automated process. Thus, there is no chance for samples to get lost or misplaced in the lab.
To know more about the sample receipt process, read here
Yes. At LifeCell, we do not reject your sample if it is tested positive for Hepatitis but it could be used only for Autologous (self) purpose.
The samples might get rejected for reasons such as low volume, blood clot, Hemolysis of cord blood, contamination & absence of growth and differentiation for cord tissue. However, such situations are very rare.
The stem cells present in the cord blood sample have no expiry date so it can be stored till the day it is required for any treatment.
Yes. The stem cells of a sibling is only a partial match while one's own stem cell sample is a 100% match in case of any stem cell transplant, so it is advisable to store the stem cells of all the children to provide 100% protection.
Yes. Twins have 2 separate umbilical cords, so it is necessary to preserve stem cells of both the cords.
Yes. You can store your baby's stem cells with LifeCell regardless of the country of birth. We can arrange express transport of cord blood samples from anywhere around the world.
In public cord blood banks, a repository of the donor units is created. Patients can look up this repository for a matching unit. If a match is found, the unit will be released to the patient for a fee. The person who donated does not have any ownership over the stem cells
Approximately a week after performing procedures such as HLA matching for cord blood & cord tissue and cell expansion that are required for the treatment.